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Tohoku Univ. Technology：High-density culture of mutant filamentous fungi: T12-060

Tohoku Univ. Technology：High-density culture of mutant filamentous fungi: T12-060

For mass fermentative production of useful substances!

Filamentous fungi have an advantage to produce a wide variety of useful substances in industry. However, it is well-known that hyphal aggregation during the liquid culture often prevents fungi to grow with high density, resulting in low productivity of useful substances. 　This invention discloses a mutant strain of a filamentous fungi, in which α-1,3-glucan synthase (AGS) gene is deficient, for high productivity of substances. 　The α-1,3-glucan in the cell wall in AGS deficient mutant (AGΔ) is significantly reduced. The AGΔ cells are dispersed well in a liquid medium and cultured with higher density compared with that of wild type. Such phenotype of AGΔ results in an increasing productivity of useful substances per unit.

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Culture medium performance testing strain (quantitative) 'EZ-Accu Shot'

Culture medium performance testing strain (quantitative) 'EZ-Accu Shot'

Easy preparation of bacterial solutions containing less than 100 CFU of viable bacteria! For those who want to reduce working time.

We would like to introduce the "EZ-Accu Shot" that we handle. It can be used for medium performance testing such as microbial limit testing methods and sterility testing methods listed in the Japanese Pharmacopoeia (JP), and we also handle strains used in medium performance testing for microbial monitoring of pharmaceutical water. In addition, we offer the "EZ-CFU One Step" with a quantity of 10 kits, as well as "EZ-CFU." Please feel free to consult us when you need assistance. 【Features】 ■ Usable for medium performance testing such as microbial limit testing methods and sterility testing methods ■ Also handles strains used in medium performance testing for microbial monitoring of pharmaceutical water ■ Usable for medium performance testing in viable count tests and specific microorganism tests ■ By dissolving the pellet in the attached hydration solution, it is easy to prepare a "microbial solution containing less than 100 CFU of viable count" *For more details, please refer to the PDF document or feel free to contact us.

  • Microbiology
  • Bacterial strains

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Tohoku Univ. Technology: Highly Dispersible Filamentous Fungi with Reduced Adhesion and Aggregation to Culture Tank Equipment: T23-061

Tohoku Univ. Technology: Highly Dispersible Filamentous Fungi with Reduced Adhesion and Aggregation to Culture Tank Equipment: T23-061

Improved in-tank environment + energy & cost-saving cultivation → Enabling high-performance, high-density culture and fermentation

Filamentous fungi represented by Aspergillus oryzae have higher production capacity of proteins and complex low-molecular compounds than bacteria and yeast, and are utilized for industrial production of a wide variety of useful substances by fermentation. However, in liquid culture, hyphae entangle and clump together, and there is a limit to high-density culture and increased production of useful substances by maximizing the space of the culture tank. Highly dispersible filamentous fungi (AGΔ-GAGΔ, see Related Inventions and Documents), which drastically reduce the viscosity of the culture solution and the formation of clumps, is a technology to solve these problems. On the other hand, even in the case of AGΔ-GAGΔ, the adhesion of the fungus to the inner structure of the culture tank, such as the inner wall of the culture tank and the agitation blade/shaft, is observed as in the case of the wild strain, and it remains to be solved in order to achieve further improvement of the productivity. The present invention relates to a new highly dispersible filamentous fungus AGΔ-GAGΔ-ΔrolA strain, in which a deletion mutation of the rolA gene encoding the surface-active protein is additionally introduced into AGΔ-GAGΔ strain. Compared with the AGΔ-GAGΔ strain, the adhesion to the inner structure of the culture tank is suppressed, and the productivity is also improved. In addition, the reduction of the agitation power and the cultivation time are also obtained by the reduction of the viscosity of the culture solution in a large-scale real machine (tank).

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[Catalog] Standard Strains from Microbiologics

[Catalog] Standard Strains from Microbiologics

Microbiologics standard strains are available for refrigerated storage and come with a third-party product guarantee.

This catalog introduces standard strains manufactured by Microbiologics, handled by Leben Japan Co., Ltd. It mainly features numerous products applicable to microbiological limit testing methods and sterility testing methods, such as "EZ-Accu Shot(TM)", "EZ-CFU One Step(TM)", and "EZ-CFU(TM)". 【Featured Products】 ■EZ-Accu Shot(TM) ■EZ-CFU One Step(TM) ■EZ-CFU(TM) ■EZ-PEC(TM) ■Epower(TM), etc. *For more details, please download the PDF or feel free to contact us.

  • Microbiology
  • Bacterial strains

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